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Taxol Separation

Analysis and Comparison of SFC and HPLC

Taxol is a tricyclic diterpenoid compound, which is the first natural secondary metabolite isolated from taxus. After clinical verification, it has good anti-tumor effects, especially on ovarian cancer, uterine cancer and breast cancer, which have a high incidence of cancers. Taxol is the most popular anticancer drug in the international market in recent years and it is considered as one of the most effective anticancer drugs in the next 20 years.

Taxol molecular formula

CAS Number: 1143-38-0

Molecular Formula:C47H51NO14

molecular weight:853.91 

There are two main impurities in taxol, which respectively are Cephalomannine (ImpurityⅠ)and 7- table -10- deacetylated paclitaxel (Impurity Ⅱ).

The structural formulas and molecules are as follows. 

1 / HPLC analysis of the challenge

Paclitaxel does not achieve baseline separation from table 7- 10- deacetylated paclitaxel and affects the determination of the content of related substances. In addition, cephalomannine and 7- table -10- deacetylated paclitaxel are the preimpurity and postimpurity of paclitaxel respectively, which is not conducive to the development of separation and purification process.

2 / SFC analysis of the challenge

Good separation of paclitaxel and related substances can be achieved by SFC method, and the baseline separation of main peaks and impurities can be completed within 25min. Meanwhile, 7-tab-10-deacetylated paclitaxel can be changed from posterior impurity to preimpurity, which is more conducive to the development of separation and purification process of paclitaxel.

3 / Characteristics of Supercritical Fluid Chromatography

Supercritical fluid chromatography (SFC) is a new separation method following GC and HPLC. It combines the ease of use of reversed-phase chromatography with the separation ability of positive-phase chromatography. In particular, it has irreplaceable advantages in chiral separation and large-scale preparation. 

l Fast Separation

SFC usually uses 3 times elution flow rate than HPLC, which makes the analysis faster and save more time.

l Less Amount of Solvent

Using Co2 and a little of modifier as mobile phase, analysis and preparation can be more green and environmental.

l Wide range of application

Not only used in small and medium polar molecules separation, as well as some of the hydrophilic macromolecular.

l High selectivity

SFC retention behavior has much difference than GC, HPLC, Which is devoted to solving the problems that traditional separation methods can not solve.