The analytical supercritical fluid chromatograph uses supercritical fluid (such as CO2) with good dissolution and mass transfer characteristics as the mobile phase, and optimizes the analysis and preparation conditions by adjusting the flow rate and composition of the mobile phase, as well as the system temperature and pressure.
Usually, a elution flow rate of 3-5 times HPLC is used, making analysis faster and preparation more time-saving;
Using CO2 and a small amount of modifier as the mobile phase for analysis and preparation is more green and environmentally friendly;
Suitable for some hydrophilic macromolecules and able to maintain biological activity;
The retention behavior differs significantly from GC and HPLC, and complements traditional separation m2ethods.
| Model | SFC-lab 20 | SFC-lab 150 |
| CO2 pump flow rate | ≤20mL/min | ≤150mL/min |
| Entrainer pump flow rate | ≤10mL/min | ≤100mL/min |
| Pressure range | ≤25MPa | ≤25MPa |
| Column oven temperature | Room temperature~70℃ | / |
| Wavelength range | 190-700nm | |
| Light source | Deuterium lamp and tungsten lamp | |
| Back pressure range | 0~25MPa | 0~20MPa |
Adapt to the specifications of chromatographic columns | 4.6mm or 10mm | 20mm or 30mm |
| Data processing unit | Greenchrom Chromatographic workstation | |